The group includes 20 uncapped players with head coach Eddie Jones set to name his matchday 23 to face the Barbarians on Thursday.
Following the Old Mutual Wealth Cup fixture England then face Argentina twice in June.
Forwards (17)
Josh Beaumont (Sale Sharks), Rob Buchanan (Harlequins), Will Collier (Harlequins), Ben Curry (Sale Sharks), Tom Curry (Sale Sharks), Tom Ellis (Bath Rugby), Charlie Ewels (Bath Rugby), Jamal Ford-Robinson (Bristol), Ellis Genge (Leicester Tigers), Ross Harrison (Sale Sharks), Nick Isiekwe (Saracens), Chris Robshaw (Harlequins), Nick Schonert (Worcester Warriors), Jack Singleton (Worcester Warriors), Will Spencer (Worcester Warriors), Sam Underhill (Bath Rugby), Mark Wilson (Newcastle Falcons).
Backs (12)
Mike Brown (Harlequins), Danny Care (Harlequins), Nathan Earle (Saracens), George Ford (Bath Rugby), Mike Haley (Sale Sharks), Sam James (Sale Sharks), Alex Lozowski (Saracens), Joe Marchant (Harlequins), Jonny May (Gloucester Rugby), Ryan Mills (Worcester Warriors), Richard Wigglesworth (Saracens), Marland Yarde (Harlequins).